Ery Vehicle p-value, n 0.25, four 0.14, four 0.32, 4 0.35, 5 Na�ve i 566 ?130 123 ?16 104 ?25 28 ?766 ?113 675 ?59 231 ?80 97 ?69 76 ?21 104 ?53 26 ?18 113 ?86 Male rat Hillslope Nav1.1 Inhibitor medchemexpress values (imply ?SEM) IV delivery Vehicle 1.9 ?0.1 1.8 ?0.1 1.9 ?0.1 1.0 ?0.two IV deliveryp-value, n 0.57, 4 0.09, three? 0.50, 4 0.67,Na�ve i 1.7 ?0.2 1.eight ?0.three two.1 ?0.four 1.five ?0.p-value, n C60 Automobile 0.40, 5 932 ?156 990 ?275 0.16, five 619 ?233 1085 ?558 0.64, 5 14 ?4 24.1 ?4.1 0.31, 5 13 ?7 23 ?16 Female rat Hillslope values (mean ?SEM) IV delivery Automobile 1.3 ?0.2 1.7 ?0.5 1.9 ?0.three 1.0 ?0.p-value, n 0.86, 5 0.46, 5 0.16, 5 0.56,p-value, n 0.95, five 0.19, 5 0.79, 5 0.67,p 0.05 versus Na�ve. iposure to IT and IV administration of C60 or car suspensions are shown in Figure 6(N = five). The linked EC50 and Hillslope values are also reported in Table three. Strain generation curves for 5-HT were not substantially diverse amongst LAD isolated from female rats exposed to IT C60 or vehicle (Fig. 6A). LAD vascular smooth muscle anxiety generation in response to 5-HT was not diverse involving LAD in the female IV C60 or IV automobile groups (Fig. 6B). Vascular smooth muscle relaxation in response to ACh was not unique in between LAD isolated from female rats exposed to IT C60 or IT car (Fig. 6C). The vascular smooth muscle relaxation response curves for ACh appeared to show a desensitization within the LAD from IV C60 exposed females, but the P2X3 Receptor Agonist medchemexpress responses have been not statistically unique from females exposed to IV vehicle (Fig. 6D). The best-fit values for the vascular smooth muscle relaxation curve for SNP in IT C60 exposed females have been drastically different than those from IT automobile exposed females (Fig. 6E) and IV exposed males (Fig. 6F).Coronary Responses to ET-1 Vascular smooth muscle contraction responses to ET-1 in LAD segments isolated from male and female rats exposed to IT or IV C60 and automobile are shown in Figure 7(N = five). Coronary artery segments isolated from male rats exposed to IT C60 showed elevated stress generation in response to ET-1 than coronary artery segments isolated from male rats exposed to IT car (Fig. 7A). Coronary artery segments isolated from male rats exposed IV to C60 or car have been not diverse involving groups (Fig. 7B). ET-1 responses in coronary artery segments isolated from female rats had been not unique in between C60 or automobile by IT instillation (Fig. 7C) or IV instillation (Fig. 7D). EC50 and Hillslope values for ET-1 concentration-response curves are reported in Table three for LAD collected from na�ve, IT i automobile and IT C60 exposed rats. Indomethacin-Sensitive Coronary Artery Responses to ET-1 The ability of Indomethacin to influence ET-1 mediated isometric stress (mN/mm2 ) generation in LAD isolated from male rats exposed to IT C60 is depicted in Figure eight(N = six). LAD seg-CARDIOVASCULAR INJURY IN RESPONSE TO CFIG. 4. Post-I/R injury serum cytokine concentrations. Serum samples were collected from male or female rats subjected to cardiac I/R injury, 24 h following intratracheal (IT) or intravenous (IV) delivery of C60 or vehicle. (A) IL-6 concentrations had been drastically higher in serum collected from male rats exposed to IV C60 compared with male IV car, male IT C60 , and female IV C60 . (B) MCP-1 concentrations were also elevated in serum collected from male rats exposed to IV C60 compared with male IV automobile, male IT C60 , and female IV C60 . (C) VEGF concentrations have been comparable in serum collected from all groups. p 0.05 by two-way ANOV.