Cum 1 I A. chroococcum 1 nd A. chroococcum 5.72 2.74 three.15 1.02 0.19 0.17 A. chroococcum A. armeniacus
Cum 1 I A. chroococcum 1 nd A. chroococcum five.72 two.74 3.15 1.02 0.19 0.17 A. chroococcum A. armeniacus HQ623182 4.47 3.12 7.83 six.40 8.30 6.60 eight.77 8.60 7.00 6.13 0.80 0.49 0.66 1.58 0.28 0.20 0.93 0.69 eight.50 40.40 45.80 eight.ten four.80 four.50 13.50 ten.10 1 I A. chroococcum 1 I A. chroococcum HQ623181 1 I A. chroococcum 1 nd A. chroococcum 1 I A. chroococcum HQ623180 rep-PCR group ARDRA cluster Species assignments Partial 16S rDNA sequence (accession number) Soil chemical parameters pH EC (mS cm-1 ) P (ppm) 7.40 51.00 1.90 7.70 8.Sampling siteMaize stubbleThe Scientific World Caspase 5 custom synthesis JournalAgricultural bareLagoon bankLagoon bankGeographical origin Buenos Aires (Azul) Buenos Aires (Balcarce) Buenos Aires (Mar Chiquita) Buenos Aires (Mar Chiquita) Buenos Aires (Santa Clara del Mar)Urban landSide of roadNatural pastureNatural pastureRiver bankBuenos Aires (Santa Clara del Mar) Chubut (Esquel) Chubut (Gaiman) Chubut (Trevel ) i Jujuy (Tilcara) AT11 AT13 AT26 AT27 AT39 AT32 II 2 nd 1 nd 1 1 1 1 nd nd nd I A. chroococcum A. chroococcum A. chroococcum A. chroococcumSide of routeaJujuy (Tilcara)Natural pastureWheat cropWheat cropWheat crop two nd IV IV ndATA. armeniacus3.six.0.11.Wheat crop three AT19 AT14 AT1 3ATA. armeniacus HQ591467 A. c-Rel list salinestris A. salinestris A. salinestris3.15 1.78 1.64 three.6.06 6.40 7.80 7.0.52 0.21 2.24 nd11.ten 48.80 three.30 127.Soybean cropNatural pastureEntre R s i (Paran a Crdoba o (Corral de Bustos) Crdoba o (Corral de Bustos) Crdoba o (Corral de Bustos) Salta (Embarcacin) o Salta (Joaqu V. i Gonz ez) a Santiago del Estero (Quimil iSoybean cropTable 1: Continued. rep-PCR group ARDRA cluster Species assignments Partial 16S rDNA sequence (accession quantity) OM ( ) Soil chemical parameters pH EC (mS cm-1 ) P (ppm)Geographical originSampling siteIsolateSoybean crop two HQ541448 HQ623179 four four five 5 5 six IV IV IV HQ623178 IV IV IV A. salinestris A. salinestris A. salinestris A. salinestris A. salinestris A. salinestrisAT2 AT16 AT17 AT3 three 3nd nd nd IV A. salinestris A. salinestris A. salinestris A. salinestris2.78 1.09 2.81 0.19 two.10 1.00 0.7.16 7.50 7.70 8.77 7.43 eight.28 8.nd 0.48 1.50 0.28 0.54 0.94 0.104.20 7.40 43.50 four.80 7.00 3.00 4.Organic pastureATRiver bankATSide of routeaATSoybean cropATSoybean cropATChubut (Puerto Madryn) Chubut (Villa Ameghino) Jujuy (Tilcara) Santa Fe (Videla) Santa Fe (Videla) Jujuy (Tilcara)Side of routeaATaBuenos Aires, Crdoba, Entre R s, and Santa Fe are provinces in the Pampas area; Jujuy, Salta, and Santiago del Estero are provinces in the Northwest area, Chubut is usually a province from Patagonia region of o i Argentina. Corresponded for the exact same soil sample. OM: organic matter; EC: electrical conductivity; P: extractable phosphorus; nd: not determined.The Scientific World JournalThe Scientific Planet JournalSimilarity ( ) 60 80 100 AT4 AT9 AT25 AT27 AT28 AT30 AT43 I II ATBNM 272 A.chroococcummAT33 NRRL B-14627A.vinelandii III NRRL B-14641A.vinelandii NRRL B-14644 A.vinelandii AT12 AT38 AT37 AT10 AT14 AT29 IV AT19 AT42 ATFigure two: Amplified ribosomal DNA restriction evaluation (ARDRA) of Azotobacter representative strains of each rep-PCR group and reference strains. The dendrogram based on analysis of restriction patterns of 16S rDNA obtained with HhaI was constructed employing the GelCompar II program and the Dice ( ) pairwise coefficient of similarity along with the UPGMA algorithm. Clusters were defined at the 80 similarity level. The cophenetic correlation value for this dendrogram was 0.95.to A. armeniacus DSM 2284T (99 identity), and t.
