Rship of your Hungarian Academy of Sciences also supported KK.OWP1.08 = LBF04.Impact of pathogenic microbes and healthful microbiota by Lactobacillus-derived extracellular vesicles Bao-Hong Lee1; Wei-Hsuan Hsu2; Tang-Long Shen1 Division of Hematology and Oncology, Division of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan, New Taipei City, Taiwan (Republic of China); 2Industrial Technology Research Institute, New Taipei City, Taiwan (Republic of China); 3Department of Plant Pathology and Microbiology, National Taiwan University, Taipei, Taiwan (Republic of China)OWP1.07 = LBS07.Function of Wnt4 exosomes in thymic ageing Krisztina Banfai1; Kitti Garai1; David Ernszt2; Judit E. Pongracz1; Krisztian KvellBackground: The prospective of Lactobacillus strains against pathogenic microbialinfection has been investigated more than previous decades. Extracellular vesicles (EVs) are membrane-based structure secreted from many microbes,which includes lactic acid bacteria. EVs serve as automobiles to carry diverse types of cellular cargo, including lipids, proteins, receptors and effector moleculesto therecipient cells. Recent research have demonstrated that Lactobacillus-derived EVs modulate microbiota, Caspase 3 Inducer Gene ID suppress cancer cells and regulate dendritic cells, whereas their detail mechanisms stay unclear. Solutions: We’ve got attempted to investigate the characteristic and antimicrobe activity of Lactobacillus-derived EVs. Results: Our data showed that under a similar development situation, the EVs production and size distribution among these 3 Lactobacillus strains, which includes Lactobacillus acidoplilus, Lactobacillus plantarum and Lactobacillus reuteri, were clearly distinct. Nevertheless, these EVs had been prominently capable of suppressing the development of pathogenic microbe Escherichia coli, Staphylococcus aureus and Vibrio parahaemolyticus. Summary/Conclusion: These final results indicated that Lactobacillusderived EVs could be applied as novel agents for keeping or regulating wholesome microbiota.OWP1.09 = LBS08.Catching the hedgehog: unravelling hedgehog secretion throughout filopodia-mediated transport Gustavo Aguilar1,two; Markus Affolter2; Isabel Guerrero1Institute of Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Pecs, Pecs, Hungary, P s, Hungary; 2Institute of Physiology, Faculty of Medicine, University of Pecs, Pecs, Hungary, P s, HungaryBackground: Wnt4 plays a vital part in advertising the improvement and halting the ageing of the thymus. For the duration of ageing Wnt4 is downregulated, when PPAR is up-regulated and triggers adipose involution. On the other hand, miR27b was described to suppress PPAR. Our goal was to prove the presence of Wnt4 in exosomes, to detect its effect and adhere to its path each in vitro and in vivo. Strategies: Exosomes were harvested from control and Wnt4 overexpressing TECs (thymic epithelial cells) for further experiments. Exosomes had been visualized by transmission electron microscopy. Exosomal miR27b CXCR4 Inhibitor MedChemExpress levels have been measured by TaqMan qPCR, although Wnt4 protein content was assayed by ELISA. DiI-labelled exosomes were applied on mouse and human thymus sections as well as iv-injected into mouse for in vivo tracking. Final results: Transmission electron microscopy showed exosomes ranging 50100 nm in size. TaqMan miRNA assay measured elevated miR27b levels, whilst ELISA showed high Wnt4-content in Wnt4-exosomes when compared with control exosomes. For functional studies steroid (Dx)-induced TECs had been employed as cellular ageing model. Dx accelerated ageing, but Wnt4-con.