Ssues exactly where the illness symptoms manifest, we examined root and leaf tissues separately, and initially sampled 18 h post-inoculation (Fig. 1A) as JAZ expression is often quickly induced by JA signals. Most JAZ genes exhibited greater inductions more than handle treatments in roots compared to leaves, exactly where expression peaked at 3 h post-inoculation, then rose once again at 48 h post-inoculation. The biggest inductions of 5- to Levalbuterol In Vitro 15-fold were observed for JAZ5, JAZ7, JAZ8, JAZ9 and JAZ10. The expression of JAZ3, JAZ4 and JAZ11 didn’t differ fromThe SALK_040835 line shows elevated JAZ7 expressionTo identify how the T-DNA inserted into the promoter of JAZ7 (Fig. 3A) in SALK_040835 impacts JAZ7 expression, we examined JAZ7 transcript levels in SALK_040835 and wild-type plants. Basal JAZ7 expression inside the roots and leaves of SALK_040835 was 10.8- and 5.4-fold larger, respectively, than those of wild-type N-Octanoyl-L-homoserine lactone Autophagy plants (Fig. 3B). This suggests SALK_040835 consists of an activation-tagged JAZActivation-tagged jaz7-1D mutant confers susceptibility to Fusarium oxysporum |Fig. 1. Differential JAZ gene expression is induced soon after F. oxysporum inoculation. Heat map of JAZ gene expression in roots or leaves of F. oxysporum inoculated wild-type plants over (A) a 18 h or (B) 2 d time-course. Expression is relative to manage treatment. JAZ3, JAZ4 and JAZ11 expression did not differ between inoculation or manage therapies and usually are not shown. Values have been determined by quantitative RT-PCR from three biological replicates consisting of pools of one hundred plants.allele. We consequently designated SALK_040835 as jaz7-1D. In the screening of over 30 plants, we were unable to isolate homozygous SALK_040835 lines suggesting jaz7-1D acts dominantly and that homozygous lines of this insertion mutant might be lethal, the latter of which we confirmed by way of detection of seed aborts in jaz7-1D siliques (Supplementary Fig. S3A). Independently, Yan et al. (2014) also lately reported SALK_040835C as a JAZ7 activation mutant and with modest stature. Progeny from two other separately isolated SALK_040835 lines also showed compact rosette size and increased susceptibility to F. oxysporum. Recent re-sequencing of SALK T-DNA insertion lines (O’Malley et al., 2014, unpublished) suggests SALK_040835 could include other insertions, and this raises the possibility that these more insertions, if confirmed, may perhaps contribute towards the jaz7-1D phenotypes. 1 insertion is proposed to be located inside the promoter of At2g47780 (rubber elongation issue protein), a single in the coding sequence of At2g47790 (GIGANTUS), and the others in intergenic regions. We therefore screened SALK_040835jaz7-1D plants by PCR for insertions in At2g47780 and At2g47790 but were unable to recognize any insertion in At2g47790, while all plants were heterozygous for the At2g47780 insertion. We also examined the Col-0 and SALK_040835C RNA sequencing data of Yan et al. (2014) to examine transcript levels of At2g47780 and At2g47790, and genes flanking the probable intergenic T-DNA insertions, but discovered no differential levels or truncated transcripts. Together, these results help the conclusion that thephenotypes observed in jaz7-1D are associated to the JAZ7 promoter insertion.A null mutation in JAZ7 doesn’t have an effect on resistance to F. oxysporumThe discovering that jaz7-1D includes an activation-tagged JAZ7 allele indicates the possibility that the elevated expression of JAZ7 may be responsible for elevated susceptibility to F. oxysporum in thi.
