Uret et al. This pceABCT operon is conserved within the similar genome context in all five Dehalobacter genomes,although considerable sequence variations within this operon exists among these 5 Dehalobacter strains,indicating that the pceABCT operon has been carried by every single Dehalobacter for some time (Figure S). Interestingly,inside the genomes of strain CF and strain DCA,this operon is positioned inside a region flanked by two direct repeats ( bp each and every) with other repeat patterns,indicating that the operon could have been acquired horizontally. These repeat patterns had been not discovered in strain PERK nor in strain E. No other proof for the presence of genomic islands or horizontal gene transfer events involving rdhA genes was located in any with the 5 Dehalobacter genomes. Horizontal gene transfer appears to play an essential role in shaping Dehalobacter genomes. As indicated previously,important sequence variations amongst the 5 genomes are located in regions A,B,and C highlighted in Figure . Area A capabilities a current insertion of a kb fragment (A) in the genome of strain CF (Figure. This area includes a comparatively low GC contentFrontiers in Microbiology www.frontiersin.orgFebruary Volume ArticleTang et alparative Dehalobacter Genome AnalysisFIGURE Three prospective genome rearrangements in between Dehalobacter restrictus strain PERK and Dehalobacter sp. strain CF. Note the blocks with identical color represent corresponding regions among the genomes of strain CF and strain PERK with higher similarity. Panel (A) shows the Mauve alignment and original GC skew profile in the two genomes. Panels (B represent the step artificial reversion Dimebolin dihydrochloride supplier situation that could explain the genome rearrangements observed among the two strains. Methods and in Panels (B,C) describe two sequence inversions,and step in Panel (D) represents a translocation. The resulting adjustments in Mauve alignments are depicted with corresponding changes in GC skew profiles shown for the proper.( and most genes involved are exceptional to strain CF. The insertion is likely related to a phage integrase (DCF_p) located at the finish of A (Figure. In Area C,which has a low GC content (strain CF has numerous phagerelated genes. Incorporation of area C (Figure into strain CF is most likely related to a sitespecific recombinase (DCF_p)located in the end of C. However,sequence C from strain DCA is kb lengthy and has a GC content material of . ,similar to the average GC content on the complete genome,and may perhaps indicate that C is native to Dehalobacter. In region B,each B (strain CF) and B (Strain DCA) possess a low GC content material,and poor sequence conservation was found between allFrontiers in Microbiology www.frontiersin.orgFebruary Volume ArticleTang et alparative Dehalobacter Genome Analysisfive Dehalobacter genomes,indicating a hypervariable area. Region D (Figure is a different massive phage elated region shared by each strain CF and DCA,but not located in strain PERK and E. Region D is kb extended and contains numerous genes encoding either phagerelated or hypothetical proteins,including a phage integrate positioned at the finish on the region. This insertion event targeted tRNAThr (DCF_r) and resulted in duplication ( bp) in the insertion site,which consists of a partial tRNAThr gene.Metabolic PotentialWe examined in detail the gene annotations of Dehalobacter to reveal metabolic potential and any variations involving the strains. General,these Dehalobacter strains are hugely similar. Genes for PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20972551 all important metabolic pathways and physiological specifications of.
