Ophenotype, which are thought to be formed as the consequence of a maturational delay in the DNT formation process (broken arrows). c The migrating transplanted cells that did not reach the above neurogenesis initiation sites may differentiate into mature neural cells (scattered neural cells: SNC, indicated by a star) or fail to differentiate and may form a UDNT (broken arrow). SNCs might also be derived from DNT. d, d’ The transplanted cells that failed to engraft into the parenchyma exhibit arrested or incomplete neural differentiation (BLT, UDNT) or mesenchymal differentiation (MES)Sugai et al. Molecular Brain (2016) 9:Page 9 ofFig. 5 Representative images of DNT, UDNT, and BLT. a, b Representative images of the DNT with zone formation around the central canal resembling cysts, which were observed in the 1231A3 NR-NSPC transplanted PD173074 cancer spinal cords 6 months after transplantation. a The red arrows indicate the cyst, blue arrows indicate the VZ, and black arrows indicate the IZ. Upper panel: H E, STEM121, hNestin, and hGFAP. (Scale = 500 m.) Lower panel: STEM121, hNestin, and hGFAP. (Captured in boxed area 1 in the first panel. Scale = 100 m.) (b) STEM121+ scattered neural cells (SNCs) observed in the MZ that formed in the rostral end of boxed area 2 in Fig. 5A are indicated by white arrows. There were many more migrating neural cells than is indicated. (Scale = 50 m.) (c) Representative histologic features of the UDNT, as observed in PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28045099 a 1201C1 EB-NSPCs transplanted spinal cord at the 12th week after transplantation. Upper panel: STEM121 and H E. (Scale = 500 m.) Lower panel: H E, hNestin, hGFAP, hKi67, Alcian Blue, and HNA. (Captured in the boxed area in the upper panel. Scale = 50 m.) (d) Representative histologic features of the BLT as observed in a 1210B2 NR-NSPC transplanted spinal cord at the 12th week after transplantation. The low power field views of the STEM121 staining is the same image in Fig. 3b. Upper panel: STEM121 and H E. (Scale = 500 m.) Lower panel: H E, hNestin, hGFAP, and hKi67. (Captured in the boxed area in the upper panel. Scale = 50 m.)Sugai et al. Molecular Brain (2016) 9:Page 10 ofTable 2 Comparison of histological classifications and the abnormalities observed in the in vitro analysis of each iPSC-NSPC lineNSPCs Abnormality in in vitro analysis Number of animals for each experiment (histology taken at 3 months/ 6 months) Spinal cord Brain 1210B2 EB-NSPCs CNV (no de novo, very few additional) 3/2 None 100 /100 (n = 3/2) 3/2 None 100 /100 (n = 3/2) 1210B2 NR-NSPCs CNV (one de novo, very few additional) 3/2 33 /0 (n = 1/0) 3/2 None 0 / 100 (n = 0/2) 100 /100 (n = 3/2) 1231A3 EB-NSPCs CNV (many de novo, some additional) 2/3 0 /33 (n = 0/1) 3/3 None 100 /67 (n = 2/2) 100 /100 (n = 3/3) 1231A3 NR-NSPCs Karyotype CNV (many de novo, many additional) 3/3 None 67 /100 (n = 2/3) 3/3 None 100 /100 (n = 3/3) 1201C1 EB-NSPCs CNV (some de novo, no additional) 3/0 None 100 /(n = 3/-) 3/3 None 100 /100 (n = 3/3) 1201C1 NR-NSPCs CNV (some de novo, no additional) 3/3 33 /67 (n = 1/2) 5/1 None 67 /33 (n = 2/1) 100 /100 (n = 5/1) 33.3 /(n = 1/-) 0 /67 (n = 0/2) 33 /0 (n = 1/0) None None None None None 100 /67 (n = 2/2) 67 /100 (n = 2/3) 33 /0 (n = 1/0) None 33 /0 (n = 1/0) None 0 /33 (n = 0/1) None 33 /0 (n = 1/0) None 67 /0 (n = 2/0) None 33 /0 (n = 1/0) None 33 /0 (n = 1/0) None Percentage of neural differentiation seen in each experiment (histology take.
