Rsed-phase column with an isocratic mobile phase. Analysis of the carotenoids utilized a diode array detector, and analysis of tocopherols used fluorescence. Retinil acetate and -tocopherol acetate were used as internal standards. The coefficients of variation inter- and intra-assays were less than 5 and 8 , respectively, in all determinations.Enzyme activities Superoxide dismutase (SOD) activity was measured in plasma using a tetrazolium salt for detection of superoxide radicals generated by xanthine oxidase and hypoxanthine. The chromophore produced has a maximal absorbance at 525 nm. One unit of SOD is defined as the amount of enzyme needed to exhibit 50 dismutation of the superoxide radical.Glutathione peroxidase (GPx) activity was measured using a kinetic colorimetric assay that measures activity indirectly by a coupled reaction with glutathione reductase. Glutathione reductase and NADPH reduce oxidized glutathione. NADPH oxidation is accompanied by a decrease in absorbance at 340 nm, and the decrease is directly proportional to the GPx activity in the sample. Both enzymatic activities were measured using commercially available assay kits (Cayman Chemical, Michigan, USA) following the methodology described by the manufacturer.Statistical analysis Data are presented as mean ?SD. Food intake (24-hr recalls), and the effects of control and HAD on biochemical determinations were analyzed by Friedman Repeated Measures Analysis of Variance on Ranks following Dunn’s post hoc test. Age, body mass index (BMI) and body fat percentages were analyzed by Student’s paired t-test. Antioxidant vitamin and mineral intake, as well as the com-Page 4 of(page number not for citation purposes)Reproductive Biology and Endocrinology 2009, 7:http://www.rbej.com/content/7/1/parison of data between the normal diet and the HAD, was analyzed by Mann-Whitney U-Rank Sum test (nonparametric data). A p < 0.05 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25636517 was considered statistically different. The SigmaStat software version 3.11 (Systat Software Inc., California, USA) was used to perform all statistical analyses.JC-1 site ResultsWomen without vs. with endometriosis: general characteristics All women with and without endometriosis who participated in this study lived in the metropolitan area of Mexico City and had low educational and socio-economical status. No differences were found between groups (data not shown).Women without vs. with endometriosis: antioxidant intake The FFQ reported that in the WWE group, the intakes of vitamin C (75 mg = 100 RDI) and copper (750 g = 100 RDI) were 446 RDI and 418 RDI, respectively. Meanwhile, intakes of vitamin A (570 g retinol equivalents = 100 SDI) and zinc (11 mg = 100 ) were 163 SDI and 178 RDI, respectively. Vitamin E intake (13 mg = 100 RDI) was 112 RDI. Only selenium (48 mg = 100 RDI) intake was below the recommended value for Mexican women and did not meet its 100 RDI.The mean age of WWE was 33.8 ?3.15, which was not statistically different from 32.70 ?2.46 years from the WEN group (p > 0.05, Student’s t test). The obstetric characteristics of WWE (median (min-max)) were the following: gravidity = 3 (1?), vaginal delivery = 1 (0?), Cesarean delivery = 1 (1?) and abortion = 0 (0?). On the other hand, WEN were nulligravida (p < 0.05, Mann-Whitney comparison WWE vs. WEN in all cases).Women with endometriosis: diet study General characteristics of women with endometriosis in the normal diet (n = 35) and HAD groups (n = 37) are described in table 2. Five women dropped o.
