the most effective one was used to knock down Src in the highly metastatic human breast cancer cell line MDA-MB-435S, a cell line in which Src activity is elevated and potentially playing an important role in maintaining its neoplastic phenotype

34. 60. Jha S, Shibata E, Dutta A Human Rvb1/Tip49 is required for the histone acetyltransferase activity of Tip60/NuA4 and for the downregulation of phosphorylation on H2AX after DNA damage. Mol Cell Biol 28: 2690700. 61. Gevry N, Chan HM, Laflamme L, Livingston DM, Gaudreau L p21 transcription is regulated by differential localization of histone H2A.Z. Genes Dev 21: 1869881. 62. Thompson JD, Higgins DG, Gibson TJ CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673680. 12 June 2011 | Volume 6 | Issue 6 | e20718 Biochemical and Functional Characterization of the Interaction between Liprin-a1 and GIT1: Implications for the Regulation of Cell Motility Claudia Asperti1., Veronica Astro1., Emanuela Pettinato1, Simona Paris1, Angela Bachi2, Ivan de Curtis1 1 Division of Neuroscience, San Raffaele Scientific Institute and San Raffaele University, Milano, 1446362 Italy, 2 Division of Genetics and Cell Biology, San Raffaele Scientific Institute, Milano, Italy Abstract We have previously identified the scaffold protein liprin-a1 as an important regulator of integrin-mediated cell motility and tumor cell invasion. Liprin-a1 may interact with different proteins, and the functional significance of these interactions in the regulation of cell motility is poorly known. Here we have addressed the involvement of the liprin-a1 partner GIT1 in liprina1-mediated effects on cell spreading and migration. GIT1 depletion inhibited spreading by affecting the lamellipodia, and prevented liprin-a1-enhanced spreading. Conversely inhibition of the formation of the liprin-a1-GIT complex by expression of liprin-DCC3 could still enhance spreading, although to a lesser extent compared to full length liprin-a1. No 1361504-77-9 biological activity cumulative effects were observed after depletion of both liprin-a1 and GIT1, suggesting that the two proteins belong to the same signaling network in the regulation of cell spreading. Our data suggest that liprin-a1 may compete with paxillin for binding to GIT1, while binding of bPIX to GIT1 was unaffected by the presence of liprin-a1. Interestingly, GIT and liprin-a1 reciprocally regulated their subcellular localization, since liprin-a1 overexpression, but not the GIT binding-defective liprinDCC3 mutant, affected the localization of endogenous GIT at peripheral and mature central focal adhesions, while the expression of a truncated, active form of GIT1 enhanced the localization of endogenous liprin-a1 at the edge of spreading cells. Moreover, GIT1 was required for liprin-a1-enhanced haptotatic migration, although the direct interaction between liprin-a1 and GIT1 was not needed. Our findings show that the functional interaction between liprin-a1 and GIT1 cooperate in the regulation of integrin-dependent cell spreading and motility on extracellular matrix. These findings and the possible competition of liprin-a1 with paxillin for binding to GIT1 suggest that alternative binding of GIT1 to either liprin-a1 or paxillin plays distinct roles in different phases of the protrusive activity in the cell. Citation: Asperti C, Astro V, Pettinato E, Paris S, Bachi A, et al. Biochemical and Functional Characterization of the Interaction between Liprin-a1 and GIT1: Implications for the Regulation of Cell Motility. PLoS ONE 6: e20757. doi:10.1371/journal.pone.0020757 Editor: Maddy Parsons, Kings College London, United Kingdom Received January

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