(A) Schematic representation of miR-383 binding internet site on the Gadd45g 39-UTR

miR-383 represses Gadd45g expression by specifically concentrating on Gadd45g 39-UTR. (A) Schematic representation of miR-383 binding website on the Gadd45g 39-UTR. Shaded texts show the conserved sequences amongst human, mouse, rat, rhesus monkey and horse. (B) Gadd45g 39-UTR sequence containing the predicted target sites was inserted into the pMIR reporter vector, instantly downstream the luciferase […]

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The next main antibodies were employed in this study for immunofluorescence microscopy and western blotting: c-myc (Sigma-Aldrich), cortactin 4F11 (Millipore

In this examine, we have investigated whether or not p53 plays a part in the regulation of PDGF-induced CDR development in RASM cells. We have found that p53 suppresses CDR formation in main and immortalized RASM cells. In addition, we have revealed that p53 functions by up-regulating PTEN, which in flip suppresses the Cdc42-N-WASP pathway.Wild-kind […]

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